Research Spotlight: Microvessel-on-Chip Fabrication for the In Vitro Modelling of Nanomedicine Transport

Our team at IMDEA look at the use of a tumour-on-a-chip device to replicate in vitro physiological microenvironments for use in onco-medicine testing and development and published their results in ACS Omega. The team shows their strategy for producing a round artificial microvessel on-a-chip device specifically to look at nanomedicine transport dynamics. The microchannels were designed to have the diameter in the range of tumour capillaries, with a semi-circular geometry, which was obtained by using a master mould to create an imprinted intermediate. The chip device was made by casting polydimethysiloxane on the intermediate mould and the artificial blood vessels were created by seeding human endothelial cells into the channels acting as a scaffold. The chip is then connected to by 3D-printed reservoirs to a pressure controller. Using high-magnification fluorescence microscopy, the dynamic interaction of the nanoparticles with the endothelium was assessed. To find out more about what they did and what they found, please take a look at the paper: Microvessel-on-Chip Fabrication for the In Vitro Modelling of Nanomedicine Transport

Fluorescence images of the HUVEC adhesion with actin (green) and nucleus (blue) covering the surface of the microchannels